Microsoft word - uv spectrophotometric assay of ceterizine
DHR International Journal Of Pharmaceutical Sciences (DHR-IJPS) ISSN: 2278-8328, Vol. 5, Issue 3, 2014 Available online http://www.doublehelixresearch.com/DHRIJPS
Double Helix Research
Original Article
UV spectrophotometric assay of Ceterizine formulations and their comparative study
Safila Naveed1*, Fatima Qamar1, Ghulam Sarwar1, Khan Usmanghani1,2 and
Muhammad Tanweer Alam1,3
1Faculty of Pharmacy of Pharmacy, Jinnah University for Women, Karachi, Pakistan
2Herbion Pakistan (Pvt.) Ltd., Karachi, Pakistan
3Drug Regularity Authority of Pakistan, Government of Pakistan, Karachi
*Corresponding Author: Safila Naveed
A rapid, simple, accurate, and economical least time consuming spectrophotometric method has been
developed for the assay of ceterizine and then compare assay of brand available in Pakistan. The assay is
based on the ultraviolet UV absorbance maxima at about 229 nm wavelength of ceterizine using
methanol as solvent. A sample of drug was dissolved in methanol to produce a solution containing
ceterizine. Similarly, a sample of ground tablets of different brand were extracted with methanol and
diluted with the same methanol. The absorbance of sample preparation was measured at 229 nm
against the solvent blank and the assay was determined by comparing with the absorbance of available
brand. The method can be applied for the routine QC quantitation of ceterizine in tablet formulation
Keywords— ceterizine, assay, UV pectrophotometry
Cetirizine is the fourth addition to a new generation of allergy medication called "non sedating"
antihistamine. These new antihistamines are classified as non-sedating agents because they cause less
sedation than their predecessors . Cetirizine displays a series of advantages over its predecessors as it is
free of both sedative and cholinergic effects and has potent antiallergic activity. However, it is more
sedating than the other non-sedating antihistamine. Cetirizine was among those drugs which were
widely prescribed in Europe for allergy before it was introduced in America.
yl]ethoxy]acetic acid dihydrochloride, dried substance is primarily acid metabolite of hydroxyzine
resulting from complete oxidation of primary alcohol moiety. It is available as a crystalline powder that
is soluble in water and insoluble in acetone and in methylene chloride1.
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There are are different methods have been reported for determination of cetirizine in drug, blood and
serum by using colorimeter and fluorimetric 2,3.Some UV methods also reported for determination of
ceterizine these methods were based on chloroform complexed formation between cetirizine with
bromocresol purple (BCP) or bromophenol blue (BPB). The system obeyed Beers Law for BCP & BPB 4-5 In
another method roxatidine was employed as the internal standard for quantification of cetirizine and
the internal standard (IS) 6.
But there is no simple method reported like this using single methanol.Our research group has done this
type of assay for different drugs.7-14
Ultra Violet visible 1601 Shimadzu double beam spectrophotometer was used for the analysis of
spectra. The solvent used for the assay was simple analytical grade methanol.
Wavelength Selection
About 100 ppm of cetirizine solution was accurately prepared in methanol . This active standard
solutions was scanned in the UV region 200-400 nm. The wavelength maxima (λmax) was observed at
229 nm and this wavelength was used for absorbance measurement.
Standard Stock solution
Accurately weighed 10 mg of cetirizine standard was transferred to a volumetric flask and add sufficient
water to produce 100 ml this is 100 ppm in 100 ml.
Sample Preparation
The four different brands of ceterizine Zyrtec, zanlan , ronex and sedil belong to pharma AGP,
Novartis,Hilton and Sami pharmaceuticals were purchased from different medical store of
Karachi,Pakistan. Each brands of ceterizine tablets have same batch number and were labeled to
conatin ceterizine 10mg per tablet. All the four brands have 5 year shelf life.
20 tablets of four different brands (Zyrtec, zanlan , ronex and sedil) from the marketed sample were
weighed and crushed uniformly with the help of a mortar and pestle. By calculating the average
weighed sample powder equivalent to 10 mg of ceterizine was transferred into a volumetric flask
containing 10mL water. The solutions were sonicated for about 5 min and than make up volume upto
100 ml with water.
After preparation of standard and tablet solutions, strength of solution 100 ppm in 100 ml absorbance
of the sample preparation and standard preparation in 1cm cell at the wavelength of maximum
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absorbance at about 229nm, using a spectrophotometer, using the blank solution. Calculate the
quantity in mg, of ceterizine per tablet.
RESULTS AND DISCUSSION:
Pharmaceutical assay of ceterizine was carried out by using UV spectrophotometer . Table-1 shows
regression equations of different brands of ceterizine.Four different brands of ceterizine Zyrtec, zanlan ,
ronex and sedil is taken and their solutions of 100ppm, 50ppm, 25,12.5ppm and6.25 ppm prepared for
linearity study. Their percent assay is calculated and regression equation is obtained to predict further
availability of drug. For linearity study I have prepared solutions of 100ppm, 50ppm, 25,12.5ppm
and6.25 ppm and three absorbances were taken. Figure 2 shows percent assay of different brands and
figure 3-6 shows linearity of different brands at level 100ppm, 50ppm, 25,12.5ppm and 6.25 ppm.
Correlation coefficient was found 1.00 for zyrtec and zanlan and 0.99 for ronex and sedil. According to
guideline it should not be less than 0.99 and results of all brands squared correlation coefficient found
within the limit.
Linear relationship was observed for different brands of ceterizine zyrtec, zanlan , ronex and sedil in the
concentration ranges of 100, 50, 25,12.5 and 6.25 ppm with correlation co efficient < 2. The correlation
coefficient 1.00 for zyrtec and zanlan and 0.99 for ronex and sedil was found.
Table 1:% Regression equations of different brands of ceterizine
Regression equations
y = 0.0261x - 0.005
y = 0.0261x - 0.005
y = 0.0262x - 0.0125
y = 0.0263x - 0.0154
Table 2:% assay of different brands of ceterizine brands
Average wt of tablet
Absorbance at 229
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Table 3:Descriptives of different brands ASSAY
Std. Deviation Std. Error 95% Confidence Interval for Minimum Maximum
Figure 1:Structure of ceterizine
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Figure 2:% assay of different brands
Figure 3:Linerity of Zyrtec
Figure 4:Linerity of Zalan
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Figure 5:Linerity of Ronex
Figure 6:Linerity of Sedil
Melwwanki MB, Seetharamappa J, Gowda BG and Sajjan AG (2001) Spectrofluorimetric
Determination of Cetirizine Hydrochloride in Pharmaceutical Preparations. Chemia Analityczna,
Walily El AFM, Korany MA, Gindy El A and Bedair MF (1998) Spectrophotometric and high
performance liquid chromatographic determination of cetirizine dihydrochloride in
pharmaceutical tablets, J. Pharm. Biomed. Anal., 17(3), 435-442.
(DHR-IJPS), Vol. 5, Issue 3, 2014 (DHR-IJPS) ISSN: 2278-8328
Uysal UD and Tunnel M (2006) Validated capillary electrophoresis study for the determination of
cetirizine in pharmaceutical forms, Journal of liquid chromatography & related technologies,
29(9-12), 1781-1792.
Gowda BG and Seetharamappa J (2001) Extractive spectrophotometric determination of
ceterizine HCl in pharmaceutical preparations, J. Pharm. Biomed. Anal, 25(5-6), 1021-1026.
Najma Sultana, M. Saeed Arayne and Hina Shamshad (2009) In vitro studies of the interaction
between cetirizine and H2 receptor antagonists using spectrophotometry and reversed-phase
high-performance liquid chromatography, Medicinal Chemistry Research, 1-13.
Choi SO, Lee SH, Hak S, Kim EJ and Choo HYP (2000) Enantioselective determination of cetirizine
in human urine by HPLC, Arch Pharmacal research, 23(2), 178-181.
Huma Dilshad, Safila Naveed and Baqir Naqvi (2013) Assay of new formulations of
isosorbide mononitrate by using uv spectrophotometer : BPJ0000115 - World
Research Journal of Medicine Volume : 1 Issue : 1, pg9-10
Huma Dilshad, Safila Naveed and Ghulam Sarwar (2014) Simple spectrophotometric
assay of available brands of Acetaminophen tablets and their comparative study
Journal of pharmacy and pharmaceutical sciences Volume 2, Issue 1,1-4
Safila Naveed, Fatima Qamar, Ghulam Sarwer., (2014) Percentage assay of
metformin in different medium using UV- spectrophotometer BPJ0000130 - World
Research Journal of Organic Chemistry Volume : 2 Issue : 1, 12-14 ISSN: 2320-3374
Safila Naveed (2014) Simple UV spectrophotometric assay of Atorvastatin API
formulation and their comparative study, Global Journal of Medical Research
Safila Naveed and Fatima Qamar (2014) A simple assay of Esomeprazole Using UV
spectrophotometer The Global Journal of Pharmaceutical Research (TGJPR) 3(2);
(DHR-IJPS), Vol. 5, Issue 3, 2014 (DHR-IJPS) ISSN: 2278-8328
Safila Naveed and Fatima Qamar (2014) Simple UV spectrophotometric assay of
Mefenamic acid International Journal of Pharma Sciences and Research (IJPSR)
Safila Naveed and Fatima Qamar (2014) Simple UV spectrophotometric assay of
Metronidazole Open Access Library Journal, 1
Safila Naveed and Amber Nawab (2014) Assay of LVFX (levofloxacin) in different formulation by UV spectroscopy IJPRDD, 1 (2), 13-16.
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