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## Ftp.ci2ma.udec.cl

MODELLING THE SPATIAL-TEMPORAL EVOLUTION OF THE 2009
A/H1N1 INFLUENZA PANDEMIC IN CHILE
URGERA, GERARDO CHOWELLB, PEP MULETC, AND LUIS M. VILLADAD
Abstract. A spatial-temporal transmission model of 2009 A/H1N1 pandemic influenzaacross Chile, a country that spans a large latitudinal gradient, is developed to characterizethe spatial variation in peak timing of the 2009 A/H1N1 influenza as a function of spatialconnectivity assumptions across Chilean regions and the location of introduction of the virusinto the country. The resulting model is a SEIR (susceptible-exposed-infected-removed)compartmental model with local diffusion and optional non-local terms to describe themigration of individuals of the S, E and R classes and the effect of a "hub region". Thismodel is used along with epidemiological data to explore the spatial-temporal progressionof pandemic influenza in Chile by assuming a range of transmission scenarios. Numericalresults indicate that this relatively simple model is sufficient to characterize the south-northgradient observed during the 2009 influenza pandemic in Chile, and that the "hub region"corresponding to the capital region plays the critical role in keeping the population wellmixed in a relatively short period of time.

1.1. Spatial-temporal variation of influenza. Increasing our understanding of the spa-tial dissemination patterns of influenza is essential for public health surveillance and theimplementation of reactive social distancing measures. Factors that have been associatedwith the spatial-temporal variation in seasonal influenza activity at the city or regional levelinclude local environmental characteristics (e.g., temperature, specific humidity [1, 2] thatenable local transmission, school cycles [3,4] whereby influenza transmission rates tend to de-cline during school breaks, as well as regional and global population mobility patterns [5–7]).

For instance, a study based on 30 years of influenza-related mortality found a significant cor-relation between influenza activity across US states and the rates of movement of people toand from their workplaces (workflows) compared with geographical distance [5]. Another
Date: January 27, 2014.

Key words and phrases. SEIR model, influenza pandemic, spatial-temporal model, peak timing, trans-
mission dynamics, hub region.

aCI2MA and Departamento de Ingenier´ıa Matem´atica, Facultad de Ciencias F´ısicas y Matem´aticas, Uni-
versidad de Concepci´
on, Casilla 160-C, Concepci´
on, Chile. E-Mail: rburger@ing-mat.udec.cl.

bMathematical and Computational Modeling Sciences Center, School of Human Evolution and Social
Change, Arizona State University, Tempe, AZ 85287, USA, and Fogarty International Center NationalInstitutes of Health, Bethesda, MD 20892, USA. E-Mail: gchowell@asu.edu.

cDepartament de Matem atica Aplicada, Universitat de Val encia, Av. Dr. Moliner 50, E-46100 Burjassot,
Spain. E-Mail: mulet@uv.es.

dCI2MA and Departamento de Ingenier´ıa Matem´atica, Facultad de Ciencias F´ısicas y Matem´aticas, Uni-
versidad de Concepci´
on, Casilla 160-C, Concepci´
on, Chile. E-Mail: lmvillada@ing-mat.udec.cl.

URGER, CHOWELL, MULET, AND VILLADA
Figure 1. (a) Regions of Chile, (b) pandemic onset (denoted by symbol I)and pandemic peak (denoted by symbol N) timing across the 15 Chilean regionssorted from north (top) to south (bottom) Chile [14].

study using influenza hospitalization records among older adult populations across US statesfound a significant gradient in the peak timing of influenza at the state level whereby westernstates tended to peak earlier than northeastern states [8]. Similarly, another study basedon weekly laboratory-confirmed influenza A from Canadian and US influenza surveillancesystems showed a slight gradient in peak timing from the southwest regions in the US tonortheast regions of Canada and the US. This study also found that regional influenza epi-demics were more synchronized across the US (3–5 weeks) compared with Canada (5–13weeks) [9].

1.2. The 2009 A/H1N1 pandemic influenza in Chile. In the context of the recent 2009A/H1N1 influenza pandemic, population contact rates linked to school cycles or interventionstrategies [10–12], demographic factors [13], local transmissibility [10, 11, 14], and globalmobility patterns, which drives the timing of a virus's seeding across countries [15], have beenassociated with the complex spatial and temporal evolution of the 2009 A/H1N1 influenza
SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
Arica y Parinacota
Table 1. Official data for population and area for each region and approx-imate longitudinal length. Note that we number regions consecutively fromnorth to south, and that these numbers do not correspond to the official Romannumerals assigned to the administrative regions of Chile.

pandemic. The 2009 A/H1N1 influenza pandemic spread as a single wave of transmissionin Chile during the winter of 2009 soon after the first cases were confirmed in Mexico andCalifornia, USA [16]. The first two cases of novel 2009A/H1N1 influenza in Chile wereconfirmed in metropolitan Santiago on May 17, 2009 [16]. However, a retrospective studybased on emergency room visit and laboratory viral surveillance conducted in southern cityof Puerto Montt, capital of Los Lagos region, suggested that this city could have experienceda faster transmission rate and earlier pandemic onset by the end of April 2009 comparedto the metropolitan area of Santiago [16]. Indeed, a recent study set in Chile showed thatthis country experienced a strong latitudinal gradient in pandemic peak timing in 2009,with southern regions experiencing earlier pandemic activity than northern regions [14], seeFigure 1. Specifically, the southernmost regions (Biob´ıo, Araucan´ıa, Los R´ıos, Los Lagos,Ays´
en, and Magallanes) peaked on average 16–39 days earlier relative to the northernmost
region (Arica y Parinacota). This geographical variation in pandemic peak timing in Chilewas found to be associated with differences in latitude and climatic conditions, with latitude,maximum temperature and specific humidity accounting for 69–80% of this variability inpeak timing [14]. This south-north gradient in pandemic peak timing reported for Chile isconsistent with a decreasing trend in transmissibility in the same direction, which was foundto be statistically associated with maximum temperature and specific humidity. This isconsistent with experimental studies suggesting that influenza transmission is more efficientunder dry and cold conditions [2, 17–21].

1.3. This contribution. Here we develop a spatial-temporal transmission model of the2009 A/H1N1 pandemic influenza across Chile, a country that spans a large latitudinal
URGER, CHOWELL, MULET, AND VILLADA
gradient, to characterize the spatial variation in peak timing of influenza as a function ofspatial connectivity assumptions across Chilean regions and the location of introduction ofthe virus into the country. We use epidemic modeling together with epidemiological data toexplore the spatial-temporal progression of pandemic influenza in Chile by assuming a rangeof transmission scenarios to investigate the robustness of the south-north gradient observedduring 2009 influenza pandemic in Chile.

The remainder of the paper is organized as follows. In Section 2, we describe the spatial-
temporal SEIR (susceptible-exposed-infected-removed) model. In particular, in Section 2.1we define its spatial version including local diffusion and, owing to the special geographyof Chile, reduce the model to one space dimension. In Section 2.2 we perform a stabilityanalysis for the case of constant diffusivity, starting from the well-known non-spatial versionof the SEIR model which is given by a system of four coupled ordinary differential equations,with the result that whether the basic reproductive ratio R0 is smaller or larger than onedecides whether the disease-free state is locally asymptotically stable or unstable. Then, inSection 2.3 we define a generalization of the model to include a so-called "hub region", whichis defined by a non-local convolution term. In Section 3 we outline the numerical scheme usedfor simulations. Then, in Section 4, we compare simulations obtained with the continuousmodel with diffusion and with/without migration via a hub region, which corresponds to theChilean capital (metropolitan) region. We compute the number of infected individuals andestimate the "peak time", when the maximum concentration of infected is observed, in eachregion . These results are compared with the peak times observed in [14]. These results arediscussed in Section 5.

2. Spatial-temporal SEIR model
Classical epidemiological models describe disease transmission on single populations of
individuals by aggregating all of the members into one of four different classes. However,these single population models rely on the strong assumption that the entire population ismixing homogeneously and they offer no information about the spread of the disease acrossregions, which is reasonable in small areas but does not well reflect reality across largegeographic regions.

2.1. Spatial domain and governing equations. We consider one country identified by abi-dimensional domain Ω ⊂
R , where x = (x, y) and x and y are latitudinal and longitudinal
coordinates, respectively, measured in kilometers. We assume that Ω is simply connectedand has a continuous, piecewise smooth boundary ∂Ω with a normal n = n(x).

We consider the classical epidemical SEIR model proposed by Kermack and McKendrick
[22] with standard incidence [23] (see also [24–29]). This model keeps track of four classes ofindividuals at time t and the location x ∈ Ω, the density of susceptibles S(x, t), the densityof exposed E(x, t) in which individuals are in the latent period, the density of infectivesI(x, t) in which individuals are infectious and the density of recovered R(x, t) that keepstrack of individuals removed from the infected compartment. We start from the followingassumptions.

SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
(1) The population is constant (without births or deaths, and the disease is assumed
non-letal), i.e.

where the population density is N = S + E + I + R.

(2) The transmission is described by local standard incidence. Thus, for each x ∈ Ω,
once an infected individual is introduced into the susceptible area and contacts asufficient number of susceptibles at time t, the fraction of new infected individualsper unit time is β(x)S(x, t)/N (x, t), where β(x) is the local transmission coefficient.

The number of new infected individuals per unit time resulting in each point x ∈ Ωat time t is β(x)S(x, t)I(x, t)/N (x, t).

(3) The number of individuals recovered from the infected class per unit time at x ∈ Ω
and at time t is γI(x, t), where γ is the rate constant for recovery, corresponding toa mean infectious period of 1/γ.

(4) Individuals of each class disperse by means of Fickian diffusion throughout Ω. Pre-
cisely, and let −dP (x)∇P be the population flux, where dP (x) ≥ 0 is the space-dependent diffusion coefficient for P ∈ {S, E, R}. Thus, individuals are assumed tomove an in undirected manner across the entire region and to contact only thoseindividuals in their immediate area. Infected individuals are not allowed to move.

(5) At any time the population in Ω is isolated.

Based on these assumptions, we obtain the following model:
+ ∇ · dS(x)∇S,
− κE + ∇ · dE(x)∇E,
∂I = κE − γI,
∂R = γI + ∇ · dR(x)∇R.

A sufficient condition for an isolated population is a zero-flux boundary condition for P ∈{S, E, I, R}, which leads to the homogeneous Neumann boundary conditions
for x ∈ ∂Ω and t > 0,
where n denotes the unit exterior normal vector to the boundary ∂Ω of Ω at position x and,as usual, ∂S/∂n = ∇S · n, etc. Thus, the total number of individuals at time t,
is actually constant, i.e., Ntot(t) = Ntot(t0) = N0, since
URGER, CHOWELL, MULET, AND VILLADA
For the particular geographic reality of countries that mainly extend in the direction of onlyone of the coordinate axes (an assumption obviously motivated by the geography of Chile,but which may include a few other places as well), a significant model reduction but withan acceptable loss of realism can be achieved if we reduce the model (2.1) to one spacedimension. Thus, we obtain the following system:
∂I = κE(x, t) − γI(x, t),
which is supplemented by initial conditions.

2.2. Stability analysis. To discuss the stability properties of (2.1), let us briefly recallwell-known results for the standard (non-spatial) SEIR model, which is recovered by settingdP ≡ 0, P ∈ {S, E, R}, in (2.1). We select the version of the model defined at position x (asby the choice of β(x)), and consider for a moment x as a parameter, i.e. we study the ODEmodel
dI(t; x) = κE(t; x) − γI(t; x),
dR(t; x) = γI(t; x).

For the model (2.3) we define the basic reproductive ratio associated with position x by
R0(x) := β(x)/γ.

If R0(x) > 1 for x ∈ Ω, then the disease-free steady state at position x is unstable so that anepidemic may potentially occur. If that happens then I(t; x) first increases to a maximumattained when S(t; x) = γN (t; x)/β(x), and then decreases to zero. On the other hand, ifR0(x) < 1 for x ∈ Ω, then the disease-free steady state at x is stable so that the disease diesout, i.e., I(t, x) decreases to zero at x ∈ Ω.

We now perform a linearized stability analysis for the one-dimensional system (2.2) with
dP (x) := d for ∈ {S, E, R} and β(x) = β. With this goal, we rewrite (2.2) in the form
SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
where u = (S, E, I, R)T, g(u) corresponds to the reactive term in the SEIR model, and
The linearized equation for a small perturbation v about an equilibrium point u(0) of
the dynamical system u0 = g(u) is obtained by substituting u = u(0) + v into (2.4) andneglecting second order terms in v. This yields the following linearized version of (2.4):
vt = g0(u(0))v + dDv.

We consider u(0) = (s0, 0, 0, 0)T with s0 > 0.

We now seek solutions of (2.6) of the form v(x, t) = z(t; ξ) exp(iξx) for a fixed frequency
ξ. The vector function z satisfies the system of ordinary differential equations
z0 = M u(0); ξz,
where we define the matrix
M u(0); ξ := g0 u(0) − ξ2dI∗,
where I∗ = diag(1, 1, 0, 1) and
The linearized asymptotical stability of u(0) is equivalent to limt→∞ z(t, ξ) = 0 for all ξ,
and this is equivalent to Re λ < 0 for any eigenvalue λ of M (u(0); ξ) and any ξ ∈ R. Theeigenvalues of M (u(0); ξ) are λ1,2 = −ξ2d and
− (γ + κ + dξ2) ± p(γ + κ + dξ2)2 − 4(κ(γ − β) + γdξ2),
so that Re λ3,4 < 0 if and only if κ(γ − β) + γdξ2 > 0. Therefore we have proved the followinglemma.

Lemma 1. The equilibrium point u(0) is locally asymptotically stable for the model (2.2) ifR0 = β/γ < 1 and unstable if R0 > 1.

2.3. Continuous model with a hub region. For certain subpopulation u ∈ {S, E, R}, aconstant non-local migration can be represented by the following equation:
K(z, x)u(z, t) dz −
K(x, z)u(x, t) dz,
where [xa, xb] is certain hub region and
a, xb] or y ∈ [xa, xb],
URGER, CHOWELL, MULET, AND VILLADA
is the function that indicates the rate of individuals at x that move to y. The first integral in(2.7) corresponds to migration from the hub region to a point x at rate ν, while the secondintegral corresponds to migration of population from x to the hub region at rate ν. Observethat
K(z, x)u(z, t) dz −
K(x, z)u(x, t) dz
u ∈ {S, E, I, R},
therefore the total population remains constant.

3. Numerical scheme
We consider the domain [0, L] of length L = 4200, which is approximately the length of
continental Chile in kilometers. For grid points xj := (j − 1 )∆x for j = 1, . . , M , where
∆x := L/M , and tn := n∆t for n ∈ N0, we calculate approximate values un ≈ u(x
u ∈ {S, E, I, R}. We denote un = (Sn, En, In, Rn)T and un = (un, . . , un )T.

The continuous model (2.2), supplied with suitable initial conditions, is solved numerically
by an explicit-implicit Euler method, for which the reaction is discretized explicitly, while animplicit discretization is used for the diffusive part in the continuous model. (This methodis the simplest variant of the so-called IMEX schemes for convection-diffusion problems,see [30].) The resulting scheme is
un+1 = un + ∆t G(un, x) + dD∆xun+1,
where G(un, x) is a vector of 4M components given by
β(x)SI/N − κE
g (S, E, I, R)T, x =
and D∆x is the (4M ) × (4M ) matrix that discretizes (2.5):
D∆x = L∆x ⊗ I∗,
I∗ = diag(1, 1, 0, 1),
where L∆x denotes the usual discretization of the 1D Laplacian with Neumann boundaryconditions on an M -grid over the spatial domain [0, L] with cell size ∆x = L/M .

The model with non-local migration (2.7) is discretized in a similar way. In this case the
non-local migration term is treated explicitly:
un+1 = un + ∆t G(un, x) + dD∆xun+1 + Hhub(un),
SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
where Hhub(un) is a vector of 4M components that approximates the integral terms in (2.7)by the midpoint rule:
(Hhub(un))i = ∆x
In the simulations we use M = 1000 and ∆t = 0.1 d, since we have checked that more
resolution does not significantly change the simulation.

4. Numerical simulations
4.1. Epidemiological and population data. We relied on a large individual-level datasetcomprising all hospitalizations for severe acute respiratory infection (hereafter referred to asSARI) reported by all public and private hospitals to the Chilean Ministry of Health during01-May to 31-December 2009 to characterize peak timing across Chilean regions.

A total of 1809 SARI hospitalizations (29.4%) were laboratory confirmed with A/H1N1
pandemic influenza. We obtained regional estimates of population size for 2009 from theInstituto Nacional de Estad´ısticas [31].

We consider the initial population according to official data to December 2011 (see [14])
and assume a constant distribution of population along its geographical localization for eachregion based on official data that are tabulated in Table 1.

4.2. Initial values and constants. It is difficult to determine suitable values for E(x, 0)and I(x, 0) that would allow us to obtain similar results as those reported in [14]. We there-fore tested four different initial conditions corresponding to different scenarios. "Scenario i"corresponds to one infected individual uniformly distributed along region i and zero infectedindividuals in all other regions. The number of individuals of class P ∈ {S, E, I, R} in regioni ∈ {1, . . , 15} at time t is computed as
where li is the latitude interval corresponding to region i. The number of individuals newlyinfected in the time interval [t − 1, t] (t measured in days) in region i is calculated as
The constants are chosen as 1/κ = 2 d and 1/γ = 4 d, κ = 0.5 d−1 and γ = 0.25 d−1.

Moreover we choose d = 10 km2/d and ν = 10−5 d−1.

URGER, CHOWELL, MULET, AND VILLADA
Figure 2. Simulation of disease spread for four different initial scenarios withd(x) = 10, ν = 1 × 10−5 and R0(x) > 1: (a) Scenario 3, (b) Scenario 7, (c)Scenario 10, (d) Scenario 13.

4.3. Case R0(x) > 1 for x ∈ [0, L]. In this case the local transmission coefficient β(x) is setsuch that R0(x) = β(x)/γ satisfies R0(0) = 1.2 (northernmost region) and increases linearlyas a function of space until R0(L) = 1.6 (southernmost region) i.e.

0 ≤ x ≤ L.

According to Lemma 1 in Section 2.2 any point in the domain is a endemic point.

In Figures 2 and 3 the disease spread for different initial scenarios is shown. In Figure 4
the peak times of simulations for different scenarios are shown and compared with the peaktiming reported in [14]. Scenarios 10 and 13 give a good fit to the experimental data, thuspointing that the epidemic might have started in southern Chile. Since the local reproductivenumber increases from southern to northern regions in our model, the attack rates shown inFigure 3 also increase in the same direction as R0.

SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
Figure 3. Simulation of disease spread for four different initial scenarios;same results as in Figure 2 but normalized by the number of inhabitants ineach region: (a) Scenario 3, (b) Scenario 7, (c) Scenario 10, (d) Scenario 13.

Finally, we display in Figure 5 the computed peak timing for the model (2.2) (without a
hub region) and the diffusion coefficient d = 1000 km2d−1, which is much larger than the oneused before and has been chosen so that the time frame of the peak timing of the epidemicresembles that of the experimental data.

4.4. Case R0(x) < 1 for x ∈ [0, L/2) and R0(x) ≥ 1 for x ∈ [L/2, L]. In this case, thelocal transmission coefficient β(x) is set so that R0(0) = 0.8 (northernmost region) and thatincreases linearly as a function of space until R0(L) = 1.2 (southernmost region), i.e.,
0 ≤ x ≤ L.

The parameters for diffusion and local-migration are d = 1000 km2d−1 and ν = 2 × 10−4 d−1.

URGER, CHOWELL, MULET, AND VILLADA
Peak times (days) 50
Peak time (days) 50
scenario 13observed data
10 11 12 13 14 15
10 11 12 13 14 15
North <== regions ==> South
North <== regions ==> South
Figure 4. Simulated region-wise peak timing for different scenarios with d =10 km2d−1, R0(x) > 1 for x ∈ [0, L], and including a hub region (region 7)with ν = 10−5d−1.

scenario 3scenario 7
scenario 10scenario 13
10 11 12 13 14 15
North <== regions ==> South
Figure 5. Simulated region-wise peak timing for different scenarios with d =1000 km2d−1, R0(x) > 1 for x ∈ [0, L], without a hub region.

In Figure 6 the newly infected individuals at each regions are shown. It is remarkable that
an epidemic in the northern regions is triggered from an epidemic from the southern regions,even when the corresponding R0 is less than one.

SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
Normalized new infected per day
Figure 6. Simulation new infected Inew(t) for each region i for scenario 3
(dashed curves) and scenario 13 (solid curves) for d = 1000 km2d−1, a hubregion with ν = 2 × 10−4 d−1, R0(x) < 1 for x ∈ [0, L/2) and R0(x) ≥ 1 forx ∈ [L/2, L].

5. Conclusions and discussion
We have used epidemic modeling to gain a better understanding of the spatial-temporal
pattern associated with the 2009 A/H1N1 influenza pandemic in Chile. Our results show thata relatively simple spatial SEIR transmission model with a single hub representing the highlyconnected metropolitan region is able to reproduce the qualitative characteristics of thespreading pandemic wave in Chile (see Figures 2 and 3). These results further support thatthe SEIR model is a suitable basis for the description of the 2009 influenza A/H1N1; see [32]for a compartmental version of this model applied to describe the spread of this disease inJapan. Moreover, our findings suggest that the south-north gradient in pandemic peakingtiming observed in Chile in 2009 is robust to variations in the initial conditions (e.g., location
URGER, CHOWELL, MULET, AND VILLADA
of initial infectious cases) as long as the local basic reproduction number follows an increasingtrend from south to north based on earlier estimations in [14]. Importantly, our resultssuggest that future influenza pandemics could follow similar spatial temporal dynamics tothat of the 2009 A/H1N1 influenza pandemic. Our findings could have implications forpandemic preparedness and control of future influenza pandemics.

Our results indicate that the hub region that corresponds to the metropolitan region of
Chile plays the critical role in keeping the entire population well mixed in a relatively shortperiod of time. Hence, the infection is quickly spread across the entire territory as soonas the initial cases are seeded in any region of the country (Figure 4). Overall, the peaktiming tended to occur first in the region where the initial cases were first introduced, but itthen rapidly spread throughout the country and was locally modulated by the correspondingtransmissibility level as measured by R0 in each region, and followed a decreasing trend ofR0 from the southernmost to the northernmost regions of Chile. Our results also showedthat even when the region-specific R0 is set to values less than one, it is possible to generatesmall outbreaks via frequent importation of exposed individuals, through the hub, from otherregions that support epidemic outbreaks, see Figure 6. In contrast to the transmission modewith hub, our transmission model with spatial local diffusion alone was not able to generatepandemic profiles that were qualitatively consistent with the 2009 A/H1N1 pandemic datafrom Chile obtained in [14]. Specifically, the results obtained using the diffusion model werequite sensitive to the initial region where the first cases were introduced as shown in Fig 5.

Perhaps not surprisingly the spatial model with diffusion generated the best fits to peaktiming data when the initial cases were introduced in the southernmost regions of Chile.

However, even in these scenarios the spreading pandemic wave took a considerable amountof time to reach the northernmost regions (over 140 days compared with about 78 days fromactual pandemic data).

It is interesting that the south-north spreading wave of 2009 pandemic activity in Chile is
reminiscent of the spread of the 2009 pandemic in Brazil, with the southernmost regions ofthis country being hit earlier and experiencing greater severity than northern regions [33]. Wehypothesize that our transmission model with a hub represented by the highly connectedareas of the south of Brazil (e.g., S˜
ao Paulo, Rio do Janeiro) and a similar south-north
gradient in transmissibility could be able to generate a qualitatively similar pattern to thatobserved in 2009 in that country. By contrast, seasonal influenza has been observed tooriginate from low-population regions in the equatorial north of Brazil and travel to highlypopulous regions in the subtropical south over a 3-month period [34], together with a weaktransmissibility gradient [35].

Although we have focused on characterizing the pandemic peak timing of the spreading
wave, the timing of pandemic onset could not be well characterized using out dataset ofsevere acute respiratory infections (SARI), which tend to capture the highest levels of theseverity pyramid. As previously reported [14], the metropolitan region experienced earlyintroductions of the A/H1N1 influenza virus in May 2009, but local outbreaks did not im-mediately followed, which suggest that local climatic conditions at the time did not enablewidespread transmission in the region. Instead, epidemiological investigations revealed thatthe well-connected southern city of Puerto Montt experienced full-scale transmission of novel
SPATIAL-TEMPORAL EVOLUTION OF THE 2009 A/H1N1 INFLUENZA PANDEMIC IN CHILE
A/H1N1 influenza as of late April 2009 before the confirmation of the first case in the coun-try [16]. This suggests that local climatic conditions played a significant role in facilitatingthe onset of the 2009 A/H1N1 influenza pandemic by modulating the timing of a shift of thebasic reproduction number from R0 < 1 to R0 > 1.

It is worth noting that we did not attempt to quantify the exact magnitude and progression
of the spread of the 2009 A/H1N1 influenza pandemic because our SARI data only allowedthe approximate identification of the timing of evolution of the pandemic (e.g. peak timing)rather than an accurate assessment of the onset, peak timing, and duration. Furthermore,the quantification of the magnitude of the pandemic in terms of attack rates would requiremore complex models than those employed here. For instance, we did not model the effectof winter vacation periods although it has been reported that the school break took placeafter the pandemic had reached peak levels in most parts of the country [14]. In addition,we did not account for the high rates of antiviral use in Chile, a country where treatmentwith oseltamivir was recommended for all symptomatic individuals older than 5 years thatcomplied with the influenza clinical case definition [16].

Our findings could have important implications for pandemic preparedness as our results
suggest that future influenza pandemics could follow similar spatial temporal dynamics tothat of the 2009 A/H1N1 influenza pandemic. Intensified surveillance strategies in southernregions could lead to earlier detection of novel influenza viruses.

RB acknowledges support by Fondecyt project 1130154; Conicyt project Anillo ACT1118
(ANANUM); Red Doctoral REDOC.CTA, MINEDUC project UCO1202 at Universidad deConcepci´
on; BASAL project CMM, Universidad de Chile and Centro de Investigaci´
Ingenier´ıa Matem´
atica (CI2MA), Universidad de Concepci´
on; and Centro CRHIAM Proyecto
Conicyt Fondap 15130015. PM is supported by Spanish MINECO project MTM2011-22741.

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Source: ftp://ftp.ci2ma.udec.cl/pub/ci2ma/pre-publicaciones/2014/pp14-03.pdf

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Microbiology (2007), 153, 1394–1404 ScbA from Streptomyces coelicolor A3(2) hashomology to fatty acid synthases and is able tosynthesize c-butyrolactones Nai-Hua Hsiao,13 Johannes So¨ding,2 Dirk Linke,2 Corinna Lange,3Christian Hertweck,3 Wolfgang Wohlleben1 and Eriko Takano13 Mikrobiologie/Biotechnologie, Eberhard-Karls-Universita¨t Tu¨bingen, Auf der Morgenstelle 28,