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Microbiology (2007), 153, 1394–1404 ScbA from Streptomyces coelicolor A3(2) hashomology to fatty acid synthases and is able tosynthesize c-butyrolactones Nai-Hua Hsiao,13 Johannes So¨ding,2 Dirk Linke,2 Corinna Lange,3Christian Hertweck,3 Wolfgang Wohlleben1 and Eriko Takano13 Mikrobiologie/Biotechnologie, Eberhard-Karls-Universita¨t Tu¨bingen, Auf der Morgenstelle 28, 72076 Tu¨bingen, Germany 2Max-Planck-Institut fu¨r Entwicklungsbiologie, Spemannstr. 35, 72076 Tu¨bingen, Germany 3Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena, Germany c-Butyrolactones play an important role in the regulation of antibiotic production and differentiationin Streptomyces. However the biosynthetic pathway for these small molecules has not yet beendetermined, and in vitro synthesis has not been reported. The function of the AfsA family of proteins,originally proposed to catalyse c-butyrolactone synthesis, has been in debate. To clarify the functionof the AfsA family, and to understand the synthesis of the c-butyrolactones, we performed insilico analysis of this protein family. AfsA proteins consist of two divergent domains, each of whichhas similarity to the fatty acid synthesis enzymes FabA and FabZ. The two predicted active sites in Received 18 November 2006 ScbA, which is the AfsA orthologue found in Streptomyces coelicolor, were mutated, and c-butyrolactone biosynthesis was abolished in all four constructed mutants, strongly suggesting Accepted 4 January 2007 that ScbA has enzymic activity.
virginiae butanolides (VBs) isolated from Streptomycesvirginiae; this synthesis is thought to involve a condensation Bacterial signalling molecules have been shown to be key of the glycerol derivative dihydroxyacetone and 7-methyl-3- regulators of differentiation, antibiotic production, plasmid oxo-octanoyl CoA to form a 7-methyl-3-oxo-octanoic acid.
conjugation, biofilm formation and pathogenicity (see In the presence of NADH, this is then converted into 6- reviews: Camilli & Bassler, 2006; Vendeville et al., 2005; dehydroVB A, and further to VB A in the presence of Venturi, 2006). c-Butyrolactones are produced by the Gram- NADPH (Sakuda et al., 1990, 1992, 1993). However, in vitro positive soil-dwelling bacteria Streptomyces, and they were synthesis proving this biosynthesis pathway has not been among the first signalling molecules to be identified reported. Recently, Shikura et al. (2002) identified a (Khokhlov et al., 1967). The first and the most well- stereospecific reductase essential for the last step in VB characterized c-butyrolactone is A-factor from Streptomyces griseus, and it is required in nanomolar concentrationsfor antibiotic (streptomycin) production and sporulation.
A putative A-factor biosynthetic gene, afsA, has been cloned Today, several c-butyrolactones are known, including the from S. griseus (Horinouchi et al., 1985), and a number of Streptomyces coelicolor butyrolactones (SCBs), which regulate homologues of AfsA have subsequently been identified from actinorhodin and undecylprodigiosin, and the recently several streptomycetes (see Takano, 2006, for a review).
identified polyketide synthase biosynthesis cluster Cpk in However, in vitro A-factor synthesis has not yet been S. coelicolor (see Takano, 2006, for a review; Yamada, 1999).
demonstrated, and little is known about how the synthesis is The first c-butyrolactone biosynthesis analysis was that of controlled. Cloning of afsA on a multi-copy plasmid leads toprecocious production of streptomycin in S. griseus, and synthesis of A-factor in several Streptomyces species that Present address: Department of Microbial Physiology, GBB, University of Groningen, Kerklaan 30, 9751NN, Haren, The Netherlands.
normally do not produce it (Horinouchi et al., 1985).
Expression of afsA in Escherichia coli also results in Full details of the construction of ScbA mutants and further MS dataare available as supplementary data with the online version of this production of biologically active c-butyrolactones; this expression is reduced in the presence of cerulenin, a fatty Abbreviations: SCB, Streptomyces coelicolor butyrolactones; VB, acid synthesis inhibitor (Ando et al., 1997). From these results, and those obtained by Sakuda et al. (1990, 1992) 2006/004432 G 2007 SGM Printed in Great Britain ScbA active sites are similar to FabA/Z outlined above, it has been concluded that AfsA on its own agar (Takano et al., 2001) was used for c-butyrolactone bioassays.
can produce A-factor from a glycerol derivative, and an a- MS agar (Kieser et al., 2000) was used to make spore suspensions, keto acid derived from fatty acid biosynthesis.
and for plating out conjugations with E. coli ET12567 containing theRP4-derivative pUZ8002 (Flett et al., 1997). R2 (Kieser et al., 2000) A contrasting view arose from studies of the gene barX. barX is and R2YE (Kieser et al., 2000) were used to observe morphologicaldifferentiation and antibiotic production of mutants.
an afsA homologue from S. virginiae that is located adjacent tobarA, which encodes the VB-binding protein. A barX-deletion Primers, PCR and DNA sequencing. All primers used in this mutant produces less virginiamycin and VBs, but the addition work are listed in Table 2. Amplification of DNA by PCR was done of VBs to the mutant does not restore virginiamycin pro- with Taq polymerase or ProofStart polymerase (Qiagen). E. coli duction (Kawachi et al., 2000), in contrast to the situation colony PCR was carried out by mixing a small amount of cellspicked from a single colony directly into the PCR reaction pre-mix- reported for the afsA mutant in S. griseus (Hara & Beppu, ture. DNA was sequenced by MWG.
1982). Furthermore, BarX enhances the stability of theinteraction between BarA and its cognate barB (barA homo- DNA and RNA manipulations. Plasmid DNA isolation, restric- logue) promoter by a protein–protein interaction that leads to tions and cloning experiments were carried out as described bySambrook et al. (1989). Streptomyces genomic DNA was isolated repression of barB expression. Thus, the authors conclude that according to Leblond et al. (1996). RNA was isolated as described by BarX does not possess enzymic activity analogous to AfsA, but Strauch et al. (1991).
that it may be involved in the regulation of VB synthesis.
Construction of ScbA mutants. The conserved glutamates E78 scbA is the afsA homologue in S. coelicolor, which is and E240 of ScbA were mutated to alanine, and the conserved argi- genetically the most well-characterized streptomycete, and nine R243 was mutated to lysine by site-directed mutagenesis using has a completely sequenced genome. scbA is located SLIM (Chiu et al., 2004), to yield pTE106, pTE108, pTE104 andpTE110. For full details of the construction of ScbA mutants, see the divergently from the c-butyrolactone-binding protein supplementary data available with the online version of this paper.
determinant scbR (Takano et al., 2001), thus exhibitingthe same gene organization as S. virginiae (see Takano, 2006, Introduction of the mutation into Streptomyces. pTE104, for a review). A deletion mutant of scbA does not produce pTE106, pTE108 and pTE110 were introduced into the methylation- any c-butyrolactones with antibiotic stimulatory activity, deficient E. coli strain ET12567 containing the RP4-derivativepUZ8002 (Paget et al., 1999), and transferred to S. coelicolor M751 and expression of scbA is absent in both scbA- and scbR- by conjugation (Flett et al., 1997); single-crossover exconjugants deletion mutants. While the expression of scbR is induced by were selected on MS containing apramycin and nalidixic acid to addition of SCB1, a c-butyrolactone, to the scbA mutant, obtain transconjugants M751 : : pTE104, M751 : : pTE106, M751 : : scbA transcription is not inducible under the same pTE108 and M751 : : pTE110, respectively. The genomic DNA of conditions (Takano et al., 2001). Thus, some direct role each strain was isolated, and plasmid integration was confirmed by for ScbA in transcriptional activation of its own gene PCR with primers JGatt B1-fwd and JGatt Pint-rev (Table 2), which appears probable, suggesting a possible regulatory function gave an amplified product of 0.8 kb, corresponding to the insertedregion (data not shown).
for ScbA. Production of SCBs is detected at transition tostationary phase, and this also coincides with the transcrip- RT-PCR was conducted using RNA isolated from tion of scbA (Takano et al., 2001).
M751 : : pSET152 (negative control: mutant complemented withvector), M751 : : pIJ6147 (positive control: mutant complemented To clarify these conflicting results, and to understand the with wt scbA), M751 : : pTE104, M751 : : pTE106, M751 : : pTE108 function of ScbA and the other members of the AfsA family, and M751 : : pTE110 grown in SMM liquid medium for 24 h (seeFig. 3a). A 2 mg quantity of RNA was used to synthesize the cDNA, we undertook an in silico analysis of the protein sequences.
and 27 ng of this cDNA was used as the template for the PCR reac- From these analyses, a number of conserved residues that are tion, as previously reported, using primers for scbA, hrdB, redQ, likely to participate in an enzymic function of ScbA were redD, actII-ORF4, cpkO, cpkE and actIII, except that the PCR condi- identified. Point mutations in these positions were created, tions in the present study were 96 uC for 5 min, then 30 cycles of and the resulting S. coelicolor strains were tested for the 96 uC for 40 s, 58 uC for 40 s, 72 uC for 40 s, and extension at 72 uC ability to produce c-butyrolactones with antibiotic stimu- for 7 min (Takano et al., 2005).
latory activity. All the point mutations tested resulted in loss HPLC-MS monitoring of c-butyrolactone formation. Extracts of antibiotic stimulatory function, indicating that ScbA is from SMMS-grown M751 : : pSET152, M751 : : pIJ6147, M751 : : indeed an enzyme involved in c-butyrolactone synthesis, pTE104, M751 : : pTE106, M751 : : pTE108 and M751 : : pTE110, and that it is structurally and functionally related to bacterial SMMS (medium only), and the standard (SCB1), were dissolved in fatty acid synthesis enzymes.
methanol. All experiments were done using an Agilent 1000 SeriesLC/MSD ion trap system. The system was operated with the electro-spray ionization source in the positive mode. HPLC conditions wereas follows: the column was an Agilent Zorbax Eclipse XDB C8 (5 mm, 15064.6 mm); the mobile phase consisted of A (water) andB (methanol); the gradient elution started with 10 % at 1 min to Bacterial strains, plasmids and growth conditions. Strains and 100 % at 17 min, and was then kept at 100 % for 5 min, flow rate plasmids used in this study are listed in Table 1. Streptomyces strains 1.0 ml min21. MS conditions: dry temperature 350 uC, nebulizer were manipulated as described by Kieser et al. (2000). E. coli was 60 p.s.i., dry gas 11 l min21, ion mode positive (MS and MS2).
grown and transformed according to Sambrook et al. (1989). SMM(Takano et al., 2001) was used for RNA isolation, isolation of c- Other methods. c-Butyrolactones were isolated and the bioassay butyrolactones, and antibiotic production determination. SMMS was conducted as described previously by Takano et al. (2001).
N.-H. Hsiao and others Table 1. Strains and plasmids used in this study Strain or plasmid Source or reference S. coelicolor A3(2)M145 Wild-type (genome sequenced) Kieser et al. (2000) In-frame deletion of scbA Takano et al. (2001) pSET integrated into H145 pSET152 integrated in M751 pIJ6147 (full-length scbA) integrated in M751 pTE104 (Glu-Ala scbA mutant) integrated in M751 pTE106 (E78A scbA mutant) integrated in M751 pTE108 (E240A scbA mutant) integrated in M751 pTE110 (Arg-Lys scbA mutant) integrated in M751 General-purpose cloning strain Sambrook et al. (1989) Strain used for conjugation between E. coli and Streptomyces MacNeil et al. (1992) Non-replicative conjugative cloning plasmid Bierman et al. (1992) Non-transmissible transfer plasmid Flett et al. (1997) PCR product cloning vector Expression vector scbA within pGEM-T-Easy vector Takano et al. (2001) scbA within pSET152 vector Takano et al. (2001) 1 kb scbA PCR product cloned into pGEM-T Easy 562 bp mutated scbA PCR product cloned into pGEM-T Easy AgeI scbA fragment of pTE101 cloned into pIJ6143 (lost 49 nt) pTE102 derivative containing full length of scbA (with 49 nt) EcoRI scbA fragment of pTE103 cloned into pSET152 pIJ6143 derivative containing E78A mutated scbA EcoRI DNA fragment of pTE105 subcloned into pSET152 NdeI/BbpI DNA fragment from pTE103 subcloned into pIJ6143 EcoRI scbA fragment of pTE107 cloned into pSET152 pIJ6143 derivative containing Arg-Lys mutated scbA EcoRI DNA fragment of pTE105 subcloned into pSET152 M145 : : pSET152, M751 : : pSET152, M751 : : pIJ6147, M751 : : pTE104, hidden Markov models (So¨ding et al., 2005), revealed that M751 : : pTE106, M751 : : pTE108 and M751 : : pTE110 were grown in ScbA consists of two domains homologous to the super- SMM liquid or on SMMS solid medium, and ethyl acetate extracts family of thioesterase/thiol ester dehydrase-isomerases were made from stationary-phase culture supernatants at OD450 1.2 defined in the SCOP database (Murzin et al., 1995). Each (the last time point in Fig. 3a). Equal amounts of the supernatantextracts were spotted onto confluent lawns of the indicator M145.
of these domains contains the described AfsA repeat as their After 48 h incubation, the antibiotic stimulatory activity was deter- most conserved part. Two members of the thioesterase/thiol mined (see Fig. 3b). Antibiotic production assay was conducted using ester dehydrase-isomerase superfamily, namely FabA (b- 1 ml samples of cells grown in 50 ml SMM, as described by Strauch hydroxydecanoyl thiol ester dehydrase) and FabZ [(3R)- et al. (1991).
hydroxymyristoyl ACP dehydrase], have several conservedactive site positions that also appear in both domains of ScbA (see below). Moreover, FabA and FabZ are functional ashomodimers whose two active sites are located at the dimer ScbA has active sites that are similar to FabA interface (Leesong et al., 1996; Kostrewa et al., 2005). This strongly suggests a similar spatial arrangement of the twodomains in ScbA. Although the overall sequence similarity of ScbA contains two AfsA repeats according to Pfam ScbA to FabA and FabZ is weak (15 and 13 % amino acid (Pfam03756) (Sonnhammer et al., 1998). These repeats identity, respectively), the similarity in the region around the show no similarity to any other proteins in the databases FabA/FabZ active site is pronounced, and, in particular, the when using standard BLAST or PSI-BLAST searches (Altschul hydrophobicity pattern is well conserved (Fig. 1).
et al., 1997). A sequence search of all proteins with knownstructure using the advanced remote homology detection The active site in FabA and FabZ is formed between the long tool HHpred, which is based on pairwise comparison of a-helix of one subunit, and a loop that is N-terminal to the ScbA active sites are similar to FabA/Z Table 2. Primers used in this work Sequence (5§R3§) *The AgeI site is underlined, and nucleotide changes are shown in upper-case type.
DThe insertion sequence is shown in bold upper-case type.
dThe nucleotide changes are shown in upper-case type.
equivalent helix of the other subunit. The residues involved well conserved in both domains of ScbA (R81, R243), and in catalysis are a conserved aspartate/glutamate located in that is situated just before the conserved glutamine, but not the helix, and a conserved histidine present in the loop present in FabA or FabZ.
region that forms the catalytic diad. A conserved glutamineforms a hydrogen bond with the carboxyl side chain of theconserved glutamate/aspartate residue, and stabilizes its Mutagenesis of the predicted active-site position (Leesong et al., 1996; Kimber et al., 2004; Kostrewa residues in ScbA: E78, E240 and R243 et al., 2005). In ScbA, the glutamates (E78 and E240) and To test the prediction that these homologous amino acids glutamines (Q82 and Q244) are perfectly conserved in both are involved in forming the active site of ScbA, the conserved domains, while the histidine residue is present only in the C- E78 and E240 were mutated to alanine, and the conserved terminal domain. In other homologues, the C-terminal R243 was mutated to lysine, in the AfsA repeat domains. E78 histidine is replaced by an arginine (H226R) (Fig. 1). This and E240 were mutated to alanine separately to yield may be related to, or the functional difference between ScbA pTE106 (E78A) and pTE108 (E240A), respectively, and and FabA/FabZ may be due to, the arginine residue that is together to yield pTE104 (EA). The conserved R243 was Fig. 1. ScbA consists of two divergent, duplicated domains that are remotely, but significantly, homologous to FabA and FabZ, and share most of their key catalyticresidues. The multiple alignment of the N- and C-terminal domains of ScbA, FabA and FabZ, and their homologues, was generated by HHsearch. Amino acids are colouredaccording to their physical–chemical properties: positive, blue; negative, red; polar, magenta; small, black; aromatic, dark green; non-polar, light green; and proline, orange.
Conserved amino acid residues are boxed. Residues in b-sheets and a-helices are marked with E and H, respectively. The black squares indicate the active sites of FabAand FabZ. The asterisk, hash and black circle indicate the probable functional residues E78, E240 and E243, respectively, that were mutated in our experiments.


ScbA active sites are similar to FabA/Z Fig. 2. Schematic map of amino acid exchanges made in ScbA. scbA, represented as an open arrow, has two AfsA repeatdomains (filled boxes) and three AgeI sites (vertical black lines). Mutated DNA sites at positions 232–234 (E78A), 718–720(E240A) and 727–729 (R243K) are indicated by vertical black lines. The primers used for mutagenesis are shown by arrows,and the mutated sites are labelled by asterisks.
mutated to yield pTE110 (R243K) (Methods; Figs 1 and 2).
the loss of two molecules of water (see supplementary Figs S1 These constructs were then introduced into the scbA and S2 available with the online version of this paper).
deletion mutant M751 by conjugation, to give strainsM751 : : pTE104, M751 : : pTE106, M751 : : pTE108 and To exclude any possibility that the mutated scbA constructs M751 : : pTE110. The original promoter was retained in all were not being correctly expressed, RT-PCR was conducted, as described in Methods. The expression of hrdB encodingthe major sigma factor for S. coelicolor was readily detectedin all samples (Fig. 3d), while the scbA transcript was All mutated ScbA strains lost the ability to detected in all the samples except M751 : : pSET152, which is produce c-butyrolactones, even though the the scbA deletion mutant with an integrated vector only mutated genes were expressed (Fig. 3d). No amplified products were detected using RNA The ability of the constructed mutants to produce SCB1 c- as a template, which suggests that there was no DNA butyrolactone was tested by bioassay, looking for the ability contamination of the RNA samples (data not shown). This of ethyl acetate extracts isolated from stationary-phase suggests that scbA was expressed in the mutants, and that the cultures of the strains to induce pigmented antibiotic mutations, and not the loss of transcription, led to the loss of production in the indicator strain M145 (as detailed in bioactive c-butyrolactone production.
Methods). The results are presented in Fig. 3(b). Asreported previously, an extract from the positive control To assess the effect of the mutations on growth and strain M751 : : pIJ6147 stimulated pigmentation; however, it antibiotic production, M751 : : pTE104, M751 : : pTE106, needed extracts that were twofold more concentrated than M751 : : pTE108 and M751 : : pTE110 were grown on several that of M145 : : pSET152. Extracts from M751 : : pTE104, different solid media (R2, SMMS, R2YE and MS) in M751 : : pTE106, M751 : : pTE108 and M751 : : pTE110 triplicate, but they showed only slight differences compared showed no antibiotic stimulatory activity, even when with wt : : pSET152 or M751 : : pSET152 (data not shown; using a twofold excess of supernatant extract compared see Discussion). The mutants were also grown on SMM with the positive control (Fig. 3b). This result indicates that liquid media at 30 uC in triplicate, and, after 18 h the amino acid replacements (E78A, E240A and R243K) in incubation, samples were taken at four time points (every these two putative active sites leads to an inability to 2 h) and after 34 h at OD450. The level of antibiotic produce active c-butyrolactones.
production of undecylprodigiosin and actinorhodin wasdifferent at each time point, and no conclusion could be These results were corroborated by analyses of the metabolic made from these results (data not shown). RT-PCR using profiles by HPLC-MS, using a synthetic reference of the undecylprodigiosin-pathway-specific activator RedD, SCB1. As shown in Fig. 3(c), only M145 : : pSET152 and and a representative biosynthesis gene, redQ, the actinor- M751 : : pIJ6147 were capable of producing SCB1, while no hodin-pathway-specific activator actII-ORF4, and a repre- related c-butyrolactones were detected in the mutants. MS sentative biosynthesis gene, actIII, the Cpk pathway-specific revealed the characteristic fragmentation that is initiated with activator CpkO, and a representative biosynthesis gene, N.-H. Hsiao and others


ScbA active sites are similar to FabA/Z Fig. 3. Amino acid exchange mutants do not produce bioactive c-butyrolactones. (a) Growth curves of parent (&), andmutants M751 : : pSET152 ($), M751 : : pIJ6147 (m), M751 : : pTE104 (.), M751 : : pTE106 (X), M751 : : pTE108 (b) andM751 : : pTE110 (c). Strains were grown in SMM liquid medium at 30 6C. At time points 18, 20, 22, 24 and 34 h, sampleswere collected to measure OD450. (b) c-Butyrolactone bioassays. Ethyl acetate extracts from M145 : : pSET152,M751 : : pSET152, M751 : : pIJ6147, M751 : : pTE104, M751 : : pTE106, M751 : : pTE108 and M751 : : pTE110 culturesupernatants were spotted onto confluent lawns of M145 on SMMS, and incubated at 30 6C for 48 h. Antibiotic stimulatoryactivity (purple halo) indicates that the strain produces active c-butyrolactones. Chemically synthesized SCB1 (0.25 ng) wasused as the positive control, and methanol and ethyl acetate were spotted as negative controls. (c) HPLC-MS profiles ofextracts from wild-type and mutants. Displayed is single ion monitoring at m/z 245. Ethyl acetate extracts from SMMS-grownM145 : : pSET152, M751 : : pSET152, M751 : : pIJ6147, M751 : : pTE110, SMMS (medium only), and chemically synthesized SCB1, were analysed by HPLC. The peak correspondingto SCB1 was seen in samples SCB1, M145 : : pSET152 and M751 : : pIJ6147, and is shown by an arrow. Sample names areindicated in the trace box. (d) Gene expression in the constructed mutants of scbA. RT-PCR using cDNA synthesized fromRNA M145 : : pSET152, M751 : : pSET152, M145 : : pIJ6147, M751 : : pTE108 and M751 : : pTE110 was conducted. Amplified products were run on an agarose gel, and the amplifiedgene products are indicated on the left. The expected sizes of the amplified products were: hrdB, 550 bp; scbA, 480 bp;redQ, 80 bp; cpkE, 95 bp; and actIII, 97 bp. All PCR was performed at 30 cycles. The template used for the positive controlfor all primers was M145 total DNA. M, 100 bp DNA ladder.
cpkE, was conducted in triplicate to determine the effect of Our results point strongly to a role for ScbA in the the mutation on the expression of these genes. The biosynthesis of SCB1.
expression levels of the three biosynthesis genes were similarin all the strains tested (Fig. 3d). The three activators Standard BLAST analysis of the AfsA family of proteins has showed varied expression levels (data not shown), which not revealed any similarity to other proteins in the database may correspond to the growth phase in which the RNA was since the sequencing of AfsA in 1985. By using the novel isolated (see Discussion).
software HHpred (So¨ding et al., 2005), we were able todetermine, for what we believe is the first time, that ScbA hasactive sites similar to fatty acid synthesis genes. The synthesisof fatty acids is performed by the type II fatty acid biosynthesis pathway in eubacteria. There are four steps in The precise mechanism of ScbA/AfsA homologues in c- the elongation cycle, which extends two carbons per cycle. In butyrolactone synthesis has been under considerable debate E. coli, the dehydration of the b-hydroxyl-ACP is performed for the last decade. Moreover, the exact biosynthetic by FabA or FabZ. Unsaturated fatty acids are produced by pathway for the c-butyrolactones is unknown, despite the isomerase FabM, utilizing the fatty acid intermediate their importance in the regulation of antibiotic production.
produced by FabZ, or directly by FabA, which has been N.-H. Hsiao and others shown to have an additional trans-2- to cis-3-decenoyl-ACPisomerase activity (Heath & Rock, 1996; Brock et al., 1967).
FabZ, on the other hand, does not exhibit an isomeraseactivity, and is involved in both saturated and unsaturatedfatty acid elongation. FabA is mainly found in Gram-negative bacteria that produce unsaturated fatty acids, whileFabZ is found in most bacteria (Kimber et al., 2004). The 3Dstructures and the active sites have been determined for bothFabA and FabZ. The active site residues determined frommutational studies are H70 and D84 for E. coli FabA(Leesong et al., 1996), and H133 and E147 for Plasmodiumfalciparum FabZ (Kostrewa et al., 2005).
We chose to mutate the conserved glutamates in the twoAfsA repeat domains of ScbA. The other active site residues,histidine, in the fatty acid synthases was not conserved in theN-terminal domain of ScbA, but only in the C-terminalregion, so this histidine was not mutated. The mutated C-terminal R243, on the other hand, was not conserved in thefatty acid synthase, but only in the AfsA repeat domains. Themutagenesis of either one or both conserved glutamateresidues, or the arginine, abolished the production of c-butyrolactones with antibiotic stimulatory activity. Thisstrongly suggests that these amino acids are important for Fig. 4. Proposed structural model of ScbA. Modelled structure ScbA activity, indicating the HHpred prediction was indeed of ScbA deduced from the homology to FabA and FabZ, with correct, and that ScbA is indeed an enzyme homologous to their duplicated ‘hot dog' fold, showing the two active catalytic FabA and FabZ. The exact enzymic role of ScbA will need sites that presumably bind fatty acid derivatives. The classic further biochemical analysis, but from the similarity of the inhibitor of FabZ is 3-decanoyl-N-acetylcysteamine, and this is active sites to FabA and FabZ, a dehydrase activity is modelled into the structure (green spacefill) to give the approxi- probable, which is consistent with the proposed biosynthesis mate arrangement for the bound substrates of ScbA. The con- model of butyrolactones (Sakuda et al., 1992).
served parts of the N- and C-terminal domains (the central a-helices and the preceding loops) are depicted in blue and dark Using the 3D structures determined for FabA and FabZ, a red, respectively. The side-chains of the conserved E78 and structure of ScbA is proposed (Fig. 4). FabA and FabZ are E240 are in cyan, the conserved Q82 and Q244 are in homodimers (Leesong et al., 1996), and their two identical magenta, and the conserved R81 and R243 are in red. Further active sites are located at the dimer interface. We can assume residues with potential functional importance are R228 (yellow) that the two domains of ScbA will arrange in a similar and D72 (orange).
fashion to form functional active sites. As the two domainshave diverged considerably (pairwise sequence identity of20 %), whereas the key residues remain unchanged (Fig. 1), genome sequence of S. avermitilis, a similar multi-domain it is probable that they catalyse similar reactions, but use gene (SAV7361), which also has a FabA-homologous domain, was found, as well as an individual FabA(SAV3654, 31 % amino acid identity to E. coli FabA) and As ScbA has similarity to the active sites of fatty acid FabZ (SAV3655, 31 % amino acid identity) homologue.
synthases, could ScbA also affect synthesis of fatty acids? However, there are no other homologues of FabA or FabZ in Streptomyces produces unsaturated fatty acids (Gesheva S. coelicolor. Instead, we have identified a FabM homologue et al., 1997), but, so far, from the S. coelicolor genome (SCO5144), which is also highly conserved in S. avermitilis sequence, no obvious type II FabZ homologue has been (SAV3120, 86 % amino acid identity to SCO5144). In identified (Bentley et al., 2002), while a distant orthologue Streptococcus pneumoniae, a FabA homologue has not been has been found in Streptomyces avermitilis (Ikeda et al., identified, but a FabM homologue that encodes a trans-2, 2003). Preliminary data suggest that S. coelicolor wild-type cis-3 decenoyl-ACP isomerase has been found, along with a and the scbA mutant produce C14, C16 and C17 unsaturated FabZ homologue (Marrakchi et al., 2002). It is probable that fatty acid synthesis in SMMS (data not shown). This result SCO5144 is involved in the isomerase activity, but it is not may exclude the possibility of ScbA involvement in clear what gene in S. coelicolor is responsible for the unsaturated fatty acids. However, the enzymes responsible dehydration of b-hydroxyacyl-ACP to produce branched for the synthesis of these fatty acids, i.e. the FabA or FabZ fatty acids.
equivalent in S. coelicolor, have not been identified. Adomain within a large multi-domain gene (SCO0127) has scbA is divergent to the c-butyrolactone receptor gene been found to have a weak similarity to FabZ. From the scbR. SCO6264, located adjacent to scbR, also affects ScbA active sites are similar to FabA/Z c-butyrolactone synthesis in S. coelicolor (T. Nihira & E.
function and the enzymic functions of ScbA, and this Takano, unpublished). This gene is homologous to barS1, strengthens the possibility that ScbA has dual function.
which is responsible for the reduction of the C-6 positionof VBs in S. virginiae (Shikura et al., 2002). barS1 is alsopositioned close to barX on the chromosome; barX is NOTE ADDED IN PROOF the scbA homologue in S. virginiae. There were 10 scbA After this manuscript was accepted, a paper describing the in homologues, including scbA and barX, found in the NCBI vitro synthesis of A-factor, a c-butyrolactone produced by S.
database. Of these genes, nine have surrounding sequences griseus, was published (Kato et al., 2007).
available for analysis, and six of these possess homologousgenes that probably encode members of the short-chaindehydrogenase family of proteins, which includes SCO6264 and the barS1 gene product. The genes adjacent to the afsA/ We thank K. Chater and A. Hesketh for critical reading of the scbA-like gene mmfL in S. coelicolor plasmid SCP1 and avaA manuscript, T. Ha¨rtner for the fatty acid analysis, and T. Nihira for in S. avermitilis, mmfH and SAV2267, respectively, are personal communication. N.-H. H. was funded by the Deutsche classified as medium-chain acyl-CoA dehydrogenases, and Forschungsgemeinschaft (TA428/1-1, 1-2).
are somewhat distant from the others, but still havehomology to dehydrogenases. The only exception to the‘rule' of having a dehydrogenase homologue in the cluster was S. griseus. This species is atypical, as the c-butyrolactone Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., receptor is at least 100 kb away from afsA, which is the scbA Miller, W. & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new homologue (Ando et al., 1997). Considering the conserva- generation of protein database search programs. Nucleic Acids Res 25, tion of the position of these dehydrogenase homologues, it is possible that these genes are also involved in c-butyrolactone Ando, N., Matsumori, N., Sakuda, S., Beppu, T. & Horinouchi, S.
synthesis, as in the case of barS1 and SCO6264. The (1997). Involvement of AfsA in A-factor biosynthesis as a key differences in the dehydrogenase family may also be related enzyme. J Antibiot 50, 847–852.
to differences in the c-butyrolactone precursor and end- Bentley, S. D., Chater, K. F., Cerdeno-Tarraga, A. M., Challis, G. L., product structures.
Thomson, N. R., James, K. D., Harris, D. E., Quail, M. A., Kieser, H. &other authors (2002). Complete genome sequence of the model We have previously shown that the scbA deletion mutant actinomycete Streptomyces coelicolor A3(2). Nature 417, 141–147.
overproduces antibiotics (Takano et al., 2001). In the Bierman, M., Logan, R., O'Brien, K., Seno, E. T., Rao, R. N. & present experiment, we were able to detect a slight difference Schoner, B. E. (1992). Plasmid cloning vectors for the conjugaltransfer of DNA from Escherichia coli to Streptomyces spp. Gene 116, in antibiotic production for all the strains using the integrative plasmid. This may be due to the integration of Brock, D. J., Kass, L. R. & Bloch, K. (1967). b-hydroxydecanoyl pSET152, which affects the production of the pigmented thioester dehydrase. II. Mode of action. J Biol Chem 242, 4432–4440.
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